This study aimed to systematically explore the effective active ingredients, key targets, and potential molecular mechanisms of Bushen Zhuanggu Granules (BSZGP) in the treatment of osteoporosis (OP) using network pharmacology. Active ingredients and corresponding targets of BSZGP were screened from the TCMSP database, while OP-related targets were obtained from the GeneCards database. A protein-protein interaction network was constructed using the STRING database to identify key targets, followed by GO and KEGG pathway enrichment analysis via the DAVID platform. Resultly, the findings suggest that BSZGP may primarily exert its effects through core active ingredients such as quercetin, luteolin, kaempferol, and beta-sitosterol, acting on key targets including TNF, IL6, AKT1, and HIF1A. These interactions likely regulate pathways such as the TNF signaling pathway, contributing to anti-inflammatory and antioxidant effects. In conclusion, this study preliminarily reveals that BSZGP treats OP through a synergistic mechanism involving "multiple components, multiple targets, and multiple pathways," providing a theoretical basis for further experimental validation and clinical application.

Li Wenyang, Zhang Yu. Exploration on the Active Ingredients and Pharmacological Mechanisms of Bushen Zhuanggu Granules in Treating Osteoporosis Based on Network Pharmacology. International Journal of Frontiers in Medicine (2026), Vol. 8, Issue 1: 1-13. https://doi.org/10.25236/IJFM.2026.080101.

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